Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables

Pesquisa Veterinária Brasileira

Rodrigo Costa Da Silva¹  Veruska Maia Da Costa¹  Fabio Hiroto Shimabukuro¹  Virgínia Bodelão Richini-pereira¹  Benedito Donizete Menozzi¹  Hélio Langoni¹ 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Higiene Veterinária e Saúde PúblicaBotucatu SP ,Brazil

关键词: Leptospira spp.;    sheep;    tissue samples;    isolation;    PCR;    slaughterhouse;    Leptospira spp.;    ovinos;    amostras teciduais;    isolamento;    PCR;    abatedouro;   

DOI  :  10.1590/S0100-736X2012000300002

来源: SciELO

【 摘 要 】

Leptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues.

【 授权许可】

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