Effects of in vitro selenium supplementation on blood and milk neutrophils from dairy cows

Pesquisa Veterinária Brasileira

Fernando N Souza¹  Maiara G Blagitz¹  Andréia O. Latorre¹  Clara S Mori¹  Maria Claudia A Sucupira¹  Alice M.m.p. Della Libera¹ 

[1] ,USP FMVZ São Paulo SP ,Brazil

关键词: Antioxidants;    bovine;    polymorphonuclear leukocytes;    mastitis;    Antioxidantes;    bovinos;    leucόcitos polimorfonucleares;    mastite;   

DOI  :  10.1590/S0100-736X2012000200014

来源: SciELO

【 摘 要 】

The study was designed to assess the effects of in vitro selenium addition on intracellular hydrogen peroxide production by neutrophils from the milk and blood of dairy cows. Blood from 10 dairy cows and 20 milk samples from five dairy cows were incubated with 0 mg (control) or 10μM of sodium selenite. Then, milk and blood neutrophils were submitted for evaluation of intracellular hydrogen peroxide production by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. The selenium status of the animals was evaluated by determination of the blood glutathione peroxidase activity. The results of the present work showed that in vitro selenium supplementation leads to an enhancement in intracellular hydrogen peroxide production, which indicates an improvement in the bactericidal effects of blood and milk neutrophils even in cows with a selenium-adequate status. Thus, the present study showed that in vitro Se supplementation leads to an enhancement in intracellular hydrogen peroxide production, indicating an improvement in the bactericidal effects of blood and milk neutrophils in cows with Se-adequate status.

【 授权许可】

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