Detection of rabies virus nucleoprotein-RNA in several organs outside the Central Nervous System in naturally-infected vampire bats

Pesquisa Veterinária Brasileira

Luiz F. P Vieira²  Sílvia R.f.g Pereira²  Aline C Galante²  Juliana G Castilho¹  Rafael N Oliveira¹  Paulo E Brandão¹  Ivanete Kotait¹ 

[1] ,Universidade Estadual do Norte Fluminense Centro de Ciências e Tecnologias Agropecuárias Hospital VeterinárioCampos dos Goytacazes RJ ,Brazil

关键词: Lyssavirus;    Desmodus rotundus;    non-neuronal organs;    rabies;    Lyssavirus;    Desmodus rotundus;    órgãos não neuronais;    raiva;   

DOI  :  10.1590/S0100-736X2011001000014

来源: SciELO

【 摘 要 】

Rabies is a neurological disease, but the rabies virus spread to several organs outside the central nervous system (CNS). The rabies virus antigen or RNA has been identified from the salivary glands, the lungs, the kidneys, the heart and the liver. This work aimed to identify the presence of the rabies virus in non-neuronal organs from naturally-infected vampire bats and to study the rabies virus in the salivary glands of healthy vampire bats. Out of the five bats that were positive for rabies in the CNS, by fluorescent antibody test (FAT), viral isolation in N2A cells and reverse transcription - polymerase chain reaction (RT-PCR), 100% (5/5) were positive for rabies in samples of the tongue and the heart, 80% (4/5) in the kidneys, 40% (2/5) in samples of the salivary glands and the lungs, and 20% (1/5) in the liver by RT-PCR test. All the nine bats that were negative for rabies in the CNS, by FAT, viral isolation and RT-PCR were negative for rabies in the salivary glands by RT-PCR test. Possible consequences for rabies epidemiology and pathogenesis are discussed in this work.

【 授权许可】

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