Serum protein concentrations, including acute phase proteins, in calves experimentally infected with Salmonella Dublin

Pesquisa Veterinária Brasileira

Daniela Gomes Da Silva¹  Péricles Ricardo Lacerda E Silva¹  Paulo César Da Silva¹  José Jurandir Fagliari¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Clínica e Cirurgia VeterináriaJaboticabal SP ,Brazil

关键词: Salmonella Dublin;    SDS-PAGE;    serum proteins;    calves;    Salmonella Dublin;    SDS-PAGE;    proteínas séricas;    bezerros;   

DOI  :  10.1590/S0100-736X2011000700001

来源: SciELO

【 摘 要 】

The aim of this study was to evaluate serum protein concentrations in calves experimentally inoculated with Salmonella Dublin. Twelve healthy 10 to 15-day-old Holstein calves were randomly allotted into two groups, control and infected with 10(8) CFU of Salmonella Dublin orally. The calves were subjected to physical evaluation and blood samples were collected shortly before administration of the bacteria and also 24, 48, 72, 96, 120 and 168 hours post-infection. The concentration of serum proteins was determined through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Thirty serum proteins ranging from molecular weight of 24,000 Da to molecular weight of 236,000 Da were detected. Serum concentrations of ceruloplasmin (125,000 Da), haptoglobin (45,000 Da), acid glycoprotein (40,000 Da) and a 34,000 Da protein were significantly increased in the experimentally infected calves, when compared with their concentrations in the control animals. Therefore, this study showed that S. Dublin infection could lead to the increase of certain serum proteins in calves.

【 授权许可】

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