Uso de PCR e sequenciamento do rDNA 16S para identificação de bactérias do gênero Staphylococcus isoladas de mastite bovina

Pesquisa Veterinária Brasileira

Carla C. Lange²  Maria A.v.p. Brito²  José R.f. Brito¹  Edna F. Arcuri²  Guilherme N. Souza²  Marco A. Machado²  Robert Domingues¹  Alessandra P.s. Salimena¹ 

[1] ,Embrapa Gado de LeiteJuiz de Fora MG ,Brasil

关键词: Coagulase-positive staphylococci (CPS);    coagulase-negative staphylococci (CNS);    intramammary infection;    Staphylococcus aureus;    S. chromogenes;    S. hyicus;    Estafilococos coagulase positivos;    estafilococos coagulase negativos;    infecção intramamária;    Staphylococcus aureus;    S. chromogenes;    S. hyicus;   

DOI  :  10.1590/S0100-736X2011000100006

来源: SciELO

【 摘 要 】

The objective of this study was to identify the species of 100 isolates of Staphylococcus from mastitis in dairy cows from herds located in the state of Minas Gerais, Brazil. PCR reactions were carried out using specific primers described previously for S. aureus (femA gene), S. intermedius (16S rDNA) and S. hyicus (16S-23S rDNA spacer region). In addition, products of amplification of variable regions of the 16S rDNA gene of the strains were sequenced. According to the results of the PCR, 83 strains were identified as S. aureus, 13 as S. intermedius, two as S. hyicus and two isolates were not identified. The sequencing of 16S rDNA was applied to 23 strains identified by PCR amplifications: six S. aureus and the strains identified as S. intermedius (n=13), S. hyicus (n=2) or not identified (n=2). The sequencing of 16S rDNA confirmed the six strains as S. aureus. The others 17 strains were identified as S. chromogenes (13 isolates) and S. hyicus (four isolates). Each sample was related to a specie according to the smallest E-value and highest similarity (> 99%). The identification of S. hyicus and S. chromogenes was accomplished only by 16S rDNA sequencing.

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