Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

Pesquisa Veterinária Brasileira

Priscilla F Gerber¹  Flávia F Pinto¹  Marcos B Heinemann¹  Zélia I.p Lobato¹ 

[1] ,Universidade Federal de Minas Gerais Escola de Veterinária Departamento de Medicina Veterinária PreventivaBelo Horizonte MG ,Brazil

关键词: Artificial insemination;    boar;    PCR;    porcine circovirus type 2;    semen;    Inseminação artificial;    varrão;    PCR;    circovírus suíno tipo 2;    sêmen;   

DOI  :  10.1590/S0100-736X2010001100004

来源: SciELO

【 摘 要 】

The dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars.

【 授权许可】

CC BY-NC   
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