Ultrastructural characterization of bovine umbilical cord blood cells

Pesquisa Veterinária Brasileira

Gustavo C Rodrigues²  Lílian J Oliveira²  Janaína M Monteiro²  Ana R De Lima²  Patricia O Gonçalez²  Francisco J Hernandez-blazquez²  Rudolf Leiser¹  José R Kfoury Jr¹ 

[1] ,Universidade de São Paulo (USP) Faculdade de Medicina Veterinária e Zootecnia Departamento de Cirurgia

关键词: Umbilical cord blood cells;    haematopoietic progenitor cells;    bovine;    ultrastructure;    Células do cordão umbilical;    células progenitoras hematopoiéticas;    bovino;    ultra-estrutura;   

DOI  :  10.1590/S0100-736X2010001000015

来源: SciELO

【 摘 要 】

The umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research.

【 授权许可】

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