An enzyme-linked immunosorbent assay (ELISA) for the detection of IgM antibodies against Leishmania chagasi in dogs

Pesquisa Veterinária Brasileira

Débora Carvalho¹  Trícia M.f.s. Oliveira¹  Cristiane D. Baldani¹  Rosangela Z. Machado¹ 

[1] ,Universidade Estadual Paulista , Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia VeterináriaJaboticabal SP ,Brazil

关键词: Leishmania chagasi;    diagnosis;    dogs;    IgM;    enzyme-linked immunosorbent assay;    Leishmania chagasi;    diagnóstico;    cães;    IgM;    ensaio imunoenzimático;   

DOI  :  10.1590/S0100-736X2009000200006

来源: SciELO

【 摘 要 】

Visceral leishmaniasis is an emergent zoonosis with an increasing number of new cases in Brazil where the domestic dog is an important parasite reservoir in the infectious cycle of Leishmania chagasi. An enzyme-linked immunosorbent assay (ELISA), based upon the use of a total soluble antigenic preparation of L. chagasi, was adapted for the detection of IgM antibodies in the serum of infected dogs. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 110 serum samples were taken from dogs in Belo Horizonte, Minas Gerais, Brazil, and examined for anti-L. chagasi IgM antibody by ELISA and indirect fluorescent antibody test (IFAT). About 25% (n=27) of all the dogs tested were found serologically positive for L. chagasi by IFAT, while 89.09% (n=98) were seropositive by ELISA. The results obtained by ELISA and IFAT were significantly different (P<0.01). The combined use of ELISA and IFAT is recommended in order to enable veterinary services to more efficiently detect canine visceral leishmaniasis.

【 授权许可】

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