Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis

Pesquisa Veterinária Brasileira

Manuela F.l. De Freitas²  Isabelle Da S. Luz¹  Vladimir Da M. Silveira-filho²  José W.p. Júnior¹  Tânia L.m. Stamford²  Rinaldo A. Mota¹  Maria J. De Sena¹  Alzira M.p. De Almeida¹  Valdir De Q. Balbino²  Tereza C. Leal-balbino¹ 

[1] ,Universidade Federal de PernambucoRecife PE ,Brazil

关键词: Staphylococcal toxins;    toxin genes;    milk;    dairy cows;    Toxinas estafilocócicas;    genes das toxinas;    leite;    vacas leiteiras;   

DOI  :  10.1590/S0100-736X2008001200010

来源: SciELO

【 摘 要 】

The present study was carried out in 11 dairy herds in four municipal districts of the rural area of the State of Pernambuco, Brazil. Out of 984 quarter milk (246 cows), 10 (1.0%) were positive for clinical mastitis, 562 (57.1%) for subclinical mastitis and 412 (41.9%) were negative. A total of 81 Staphylococcus spp. isolates were obtained from milk samples from the cows diagnosed with subclinical mastitis. From these, 53 (65.0%) were S. aureus, 16 (20.0%) coagulase-positive staphylococci (CPS) and 12 (15.0%) coagulase-negative staphylococci (CNS). The isolates were further investigated for the presence of toxin genes by multiplex and uniplex PCR. The main gene observed was seg followed by seh, sei and sej. The distribution of these observed genes among the isolates obtained from different areas showed a regional pattern for the SEs. The presence of toxin genes in the strains isolated from bovine milk demonstrates a potential problem for public health.

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