Pharmacological immunomodulation enhances peripheral nerve regeneration

Pesquisa Veterinária Brasileira

Ana Paula Inoe²  Francisco Carlos Pereira¹  Angelo João Stopiglia¹  Ciro Ferreira Da-silva¹ 

[1] ,Universidade ParanaenseUmuarama PR ,Brazil

关键词: N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP;    nerve regeneration;    drug effect;    sciatic nerve;    surgery;    N-acetilmuramil-L-alanil-D-isoglutamina MDP;    regeneração nervosa;    efeitos de drogas;    nervo ciático;    cirurgia;   

DOI  :  10.1590/S0100-736X2007000900002

来源: SciELO

【 摘 要 】

To assess the effect of N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP topically administrated on the regenerating peripheral neurons, twelve male C57BL/6J adult mice were equally distributed into three groups. Four mice underwent unilateral sciatic nerve transection and polyethylene tubulization, with a 4mm gap between the proximal and distal nerve stumps and were implanted with collagen + PBS (COL). Other four animals underwent the same surgical procedure but received collagen + MDP (COL/MDP) inside the prosthesis. Four animals were not operated and served as control group (NOR). After 4 weeks, the regenerated nerve cables were processed for total myelinated axon counting and myelinated fiber diameter measurement. The L5 dorsal root ganglion (DRG) was also removed and sectioned for sensory neurons counting and measurement. The results revealed significant difference (p<0.05) in axonal counting among the groups NOR (4,355±32), COL (1,869±289) and COL/MDP (2,430±223). There was a significant reduction in the axonal diameter in the operated groups (COL=3.38µm±1.16 and COL/MDP=3.54µm±1.16) compared to NOR (6.19µm±2.45). No difference was found in the number of DRG neurons between the experimental groups (COL=564±51; COL/MDP=514±56), which presented fewer sensory neurons compared to NOR (1,097±142). Data obtained indicate that locally applied MDP stimulates peripheral nerve regeneration in mice.

【 授权许可】

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