Pathogenicity of Rhodococcus equi in mice, isolated from environment, human and horse clinical samples

Pesquisa Veterinária Brasileira

Mateus M. Costa¹  Sérgio A. Machado²  Cristina C. Krewer¹  Márcia R.s. Ilha²  Dominguita L. Graça²  Ana L.m. Guaraldi²  Agueda C. Vargas² 

[1] ,UFRGS Centro de Biotecnologia Porto Alegre RS

关键词: Rhodococcus equi;    mice;    virulence;    cyclophosphamide;    Rhodococcus equi;    camundongos;    virulência;    ciclofosfamida;   

DOI  :  10.1590/S0100-736X2006000300007

来源: SciELO

【 摘 要 】

Rhodococcus equi is a facultative intracellular pathogen associated with bronchopneumonia, mesenteric lymphadenitis and enterocolitis in foals. Although R. equi is likely to be found in every horse-breeding farm, the clinical disease is unrecognized in most of them. Capsule components, equi factor, micolic acid and some products encoded by the large 85-90Kb plasmid were described as virulence factors. However, the pathogenesis of R. equi infections and the sensibility of foals are not completely understood. The aim of this study was evaluate the virulence of R. equi isolated from human, horses and environment for mices. Nine strains carrying the 85-90Kb plasmid isolated from foal clinical specimens, one from immunodeficient human patient and six plasmidless strains (four isolated from feces, one from pasture and one from immunodeficient human patient) were inoculated in cyclophosphamide immunossuppressed mice. The pathological changes and viability of R. equi cells in the liver of mice was verified after the 3rd, 6th an 10th day after inoculation for horse and environmental isolates and for R. equi isolates from human patients on the 1st, 3rd and 6th day. During the necropsy procedures, infiltrate of macrophages and pyogranulomatous lesions were detected after the sixth pos-inoculation day in the liver and spleen. In horse isolates, only plasmid positive strains were virulent, but in human isolates both strains (plasmid positive e plasmid negative) were virulent. Both groups of the immunossupressed mice inoculated with R. equi isolated from environment showed pathological changes. All R. equi strains were unable to kill non imunossuppressed mice.

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