Imunofluorescência utilizando isolados brasileiros no diagnóstico sorológico de infecção por lentivírus em caprinos

Pesquisa Veterinária Brasileira

Dilmara Reischak²  Ana Paula Ravazzolo¹  Valéria Moojen² 

[1] ,UFRGS Faculdade de Veterinária Porto Alegre RS

关键词: Small ruminant lentiviruses;    Brazilian isolates;    diagnosis;    indirect immunofluorescence;    immunodiffusion;    goats;    Lentivírus de pequenos ruminantes;    isolados brasileiros;    diagnóstico;    imunofluorescência indireta;    imunodifusão em gel de ágar;    caprinos;   

DOI  :  10.1590/S0100-736X2002000100003

来源: SciELO

【 摘 要 】

Small ruminant lentiviruses (SRLV) are distributed worldwide and cause persistent infections in sheep and goats. The purpose of this work was to develop an indirect immunofluorescent antibody (IFA) test using Brazilian SRLV isolates for serological diagnosis of infection in goats. The IFA test was compared in its sensitivity and specificity to the AGID test in which Maedi-Visna WLC-1 strain was used as antigen. Ovine synovial secondary cell cultures infected with two goat SRLV isolates (CAEV UFRGS/Br-2 and CAEV UFRGS/Br-5) were used for the IFA. Goat serum samples (n = 239) were tested by the two tests. The AGID test detected antibodies in 129 (53.9%) serum samples. A higher number of animals was considered positive for the IFA. However, different results were obtained depending on the isolate used for the antigen preparation. When CAEV UFRGS/Br-2 was used as antigen, 216 (90.3%) serum samples tested positive, against 213 (89.1%) with CAEV UFRGS/Br-5. There was no significant statistical difference between the antigens prepared with these two isolates. The IFA had sensitivity of 94.6% and 96.9%, and specificity of 14.5 % and 20%, when CAEV UFRGS/Br-2 and CAEV UFRGS/Br-5 were used as antigens, respectively. These results demonstrate that the IFA with antigens prepared with Brazilian SRLV isolates may play an important a role as a complementary serological test for the diagnosis of infections by these agents.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License