Comparison of nuclear grade and immunohistochemical features in situ and invasive components of ductal carcinoma of breast

Revista Brasileira de Ginecologia e Obstetrícia

Fernando Nalesso Aguiar²  Henrique Nogueira Mendes¹  Carlos E. Bacchi¹  Filomena Marino Carvalho¹ 

[1] ,Instituto do Câncer do Estado de São PauloSão Paulo SP ,Brazil

关键词: Breast neoplasms;    Carcinoma;    ductal;    breast;    Immunoenzyme techniques;    Receptors;    estrogen;    Receptor;    erbB-2;    keratin-5;    keratin-6;    Ki-67 antigen;    Tumor makers;    biological;    Neoplasias da mama;    Carcinoma ductal de mama;    Técnicas imunoenzimáticas;    Receptores estrogênicos;    Receptor;    erbB-2;    Queratina-5;    Queratina-6;    Antígeno Ki-67;    Marcadores biológicos de tumor;   

DOI  :  10.1590/S0100-72032013000300002

来源: SciELO

【 摘 要 】

PURPOSE:To compare the prognostic and predictive features between in situ and invasive components of ductal breast carcinomas. METHODS:We selected 146 consecutive breast samples with ductal carcinoma in situ (DCIS) associated with adjacent invasive breast carcinoma (IBC). We evaluated nuclear grade and immunohistochemical expression of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), cytokeratin 5/6 (CK5/6), and epidermal growth factor receptor (EGFR) in both components, in situ and invasive, and the Ki-67 percentage of cells in the invasive part. The DCIS and IBC were classified in molecular surrogate types determined by the immunohistochemical profile as luminal (RE/PR-positive/ HER2-negative), triple-positive (RE/RP/HER2-positive), HER2-enriched (ER/PR-negative/HER2-positive), and triple-negative (RE/RP/HER2-negative). Discrimination between luminal A and luminal B was not performed due to statistical purposes. Correlations between the categories in the two groups were made using the Spearman correlation method. RESULTS:There was a significant correlation between nuclear grade (p<0.0001), expression of RE/RP (p<0.0001), overexpression of HER2 (p<0.0001), expression of EGFR (p<0.0001), and molecular profile (p<0.0001) between components in situ and IBC. CK 5/6 showed different distribution in DCIS and IBC, presenting a significant association with the triple-negative phenotype in IBC, but a negative association among DCIS. CONCLUSIONS: Our results suggest that classical prognostic and predictive features of IBC are already determined in the preinvasive stage of the disease. However the role of CK5/6 in invasive carcinoma may be different from the precursor lesions.

【 授权许可】

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