Liver regeneration with l-glutamine supplemented diet: experimental study in rats

Revista do Colégio Brasileiro de Cirurgiões

Cibelle Ribeiro Magalhães¹  Osvaldo Malafaia¹  Orlando Jorge Martins Torres¹  Luciane Bugmann Moreira¹  Silvia Carmen Da Silva Gonçalves Tefil¹  Matheus Da Rocha Pinherio¹  Bruna Ayumi Harada¹ 

关键词: Evaluation;    Hepatectomy;    Liver regeneration;    Diet;    Glutamine;    Avaliação;    Hepatectomia;    Regeneração hepática;    Dieta;    Glutamina;   

DOI  :  10.1590/S0100-69912014000200008

来源: SciELO

【 摘 要 】

OBJECTIVE: To assess liver regeneration in rats after 60% hepatectomy with and without supplementation of L-glutamine through liver weight changes, laboratory parameters and histological study.METHODS: 36 male rats were divided into two groups: glutamine group and control group. Each group was subdivided into three subgroups, with death in 24h, 72h and seven days. The glutamine group received water and standard diet supplemented with L-glutamine, and the control recieved 0.9% saline. In all subgroups analysis of liver regeneration was made by the Kwon formula, study of liver function (AST, ALT, GGT, total bilirubin, indirect and indirect bilirubin and albumin) and analysis of cell mitosis by hematoxylin-eosin.RESULTS: In both groups there was liver regeneration by weight gain. Gamma-GT increased significantly in the control group (p < 0.05); albumin increased in the glutamine group. The other indicators of liver function showed no significant differences. Histological analysis at 72h showed a higher number of mitoses in the glutamine group, with no differences in other subgroups.CONCLUSION: Diet supplementation with L glutamine is beneficial for liver regeneration.

【 授权许可】

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