RAPD analysis of genetic variability in a multiprovenance base population of Eucalyptus grandis hill ex maiden

Revista Árvore

Susi Meire Maximino Leite²  Édson Seizo Mori¹  Celina Ferraz Do Valle¹  César Augusto Valencise Bonine¹  Celso Luís Marino¹ 

[1] ,Universidade de Marília Centro de Ciências Agrárias

关键词: Molecular markers;    forest tree breeding;    population genetics;    Marcadores moleculares;    melhoramento genético florestal;    genética de populações;   

DOI  :  10.1590/S0100-67622008000600001

来源: SciELO

【 摘 要 】

This study aimed to evaluate the genetic variability among individuals of a base population of Eucalyptus grandis and to build a molecular marker database for the analyzed populations. The Eucalyptus grandis base population comprised 327 individuals from Coff's Harbour, Atherton and Rio Claro. A few plants came from other sites (Belthorpe MT. Pandanus, Kenilworth, Yabbra, etc.). Since this base population had a heterogeneous composition, the groups were divided according to geographic localization (latitude and longitude), and genetic breeding level. Thus, the influence of those two factors (geographic localization and genetic breeding level) on the genetic variability detected was discussed. The RAPD technique allowed the evaluation of 70 loci. The binary matrix was used to estimate the genetic similarity among individuals using Jaccard's Coefficient. Parametric statistical tests were used to compare within-group similarity of the means. The obtained results showed that the base population had wide genetic variability and a mean genetic similarity of 0.328. Sub-group 3 (wild materials from the Atherton region) showed mean genetic similarity of 0.318. S.P.A. (from Coff's Harbour region) had a mean genetic similarity of 0.322 and was found to be very important for maintenance of variation in the base population. This can be explained since the individuals from those groups accounted for most of the base population (48.3% for it). The base population plants with genetic similarity higher than 0.60 should be phenotypically analyzed again in order to clarify the tendency of genetic variability during breeding programs.

【 授权许可】

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