Development and characterisation of microsatellite markers for the fungus Lasiodiplodia theobromae

Summa Phytopathologica

José Emilson Cardoso²  Michael James Wilkinson¹ 

[1] ,Embrapa Agroindústria TropicalFortaleza CE

关键词: simple sequence repeat;    molecular marker;    seqüência simples repetida (SSR);    marcador molecular;   

DOI  :  10.1590/S0100-54052008000100010

来源: SciELO

【 摘 要 】

Lasiodiplodia theobromae is an important fungal pathogen of higher plants from tropical and sub-tropical regions. The fungus infects divergent hosts in a wide range of environmental conditions, suggesting that it is highly variable. The aim of this study was to develop new polymorphic microsatellite markers from a Brazilian isolate of L. theobromae that can be used in population studies of this and related fungi. The nine microsatellite markers developed included six that revealed allelic polymorphisms among nine isolates of the disease collected from infected plants in Brazil. Preliminary evaluation of the markers suggested substantial genetic variability among Brazilian L. theobromae populations. These markers have potential utility for evolutionary and epidemiologic studies of this fungus.

【 授权许可】

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