Pseudomonas syringae pv. tabaci in papaya seedlings

Summa Phytopathologica

Luís Otávio S. Beriam¹  Irene M.g. Almeida¹  Suzete A.l. Destéfano¹  Eunice Grabert¹  Denise M. Balani¹  Mariana Ferreira¹  Júlio Rodrigues Neto¹ 

[1] ,Instituto BiológicoCampinas SP ,Brazil

关键词: papaya bacterial diseases;    16S-23S rDNA;    bacteriose do mamoeiro;    16S-23S DNAr;   

DOI  :  10.1590/S0100-54052006000100003

来源: SciELO

【 摘 要 】

The natural occurrence of Pseudomonas syringae pv. tabaci causing leaf spot symptoms in papaya seedlings is reported. The pathogen was identified through biochemical, physiological, serological, and molecular assays and artificial inoculations in papaya plants. It was also shown that the strains were pathogenic to bean and tobacco plants. The restriction patterns obtained with Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sau 3A I and Taq I of the PCR-RFLP of 16S-23S DNAr were identical to the P. s. pv. tabaci patterns. Primers corresponding to hrpL gene of P. syringae were also tested and the results grouped the papaya strains with P s. pv. tabaci. Bacterial strains were deposited at Coleção de Culturas IBSBF, Instituto Biológico, Campinas, Brazil, under access numbers 1687 and 1822.

【 授权许可】

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