Simultaneous determination of four phenolic compounds in extracts of aerial parts of Ipomoea pes-caprae (L.) R. Br. (Convolvulaceae) by HPLC-UV

Química Nova

Daniela Maes Dutra¹  Cristiane Da Silva Barth¹  Luciana Catia Block¹  Nara Lins Meira Quintão¹  Angélica Garcia Couto¹  Valdir Cechinel Filho¹  Tania Mari Bellé Bresolin¹ 

[1] ,Universidade do Vale do Itajaí Núcleo de Investigações Químico-Farmacêuticas Itajaí SC ,Brasil

关键词: Ipomoea pes-caprae;    HPLC-UV;    stability-indicating method;    analytical validation;    isoquercitrin;   

DOI  :  10.5935/0100-4042.20140237

来源: SciELO

【 摘 要 】

Among other applications, Ipomoea pes-caprae is popularly used to treat jellyfish stings, supporting the development of a product for dermatological use. Hydroethanolic spray-dried extract was chosen for the further development of phytomedicines, and a stability-indicative HPLC-UV method was developed and validated for the determination of isoquercitrin and isochlorogenic acids A, B and C. The method was developed using a C18 column (250 x 4.6 mm, 5 µm) with an acetonitrile:water mobile phase at pH 3.0 in a gradient run. The four constituents and other unidentified components of the extract were appropriately resolved without interference of degradation products after stress tests (acid, alkali, neutral, oxidant, photolysis). The method showed linearity in the isoquercitrin concentration range from 5.0-50.0 µg mL-1, with adequate precision (RSD% < 2.5% for the intra- and inter-day studies), accuracy (recovery of 100.0 ± 2.0%), and robustness. Both the herbal drug and spray-dried extract of I. pes-caprae were subjected to stability studies in accelerated and long-term conditions over four months. The samples maintained their characteristics and marker contents (< 10% of variation).

【 授权许可】

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