Química Nova | |
A fluorescence-based assay for octreotide in kinetic release from depot formulations | |
Luiz Henrique Guerreiro1  Wendell Girad-dias1  Kildare R. De Miranda1  Luís Mauricio T. R. Lima1  | |
[1] ,Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Rio de Janeiro RJ ,Brasil | |
关键词: octreotide; fluorescamine; controlled release; | |
DOI : 10.1590/S0100-40422012000500029 | |
来源: SciELO | |
【 摘 要 】
Here we report the validation of a derivatization method that makes use of fluorescamine as a selective reactant for the quantitative analysis of peptide and protein drugs in the dissolution profile from depot formulations. Typical current methods require separation of the nano/microparticles and time-consuming chromatographic runs. In this study we report a method which can be conducted without the need for complete physical separation of the particles or removal of the unreacted probe. This method was used here for the analysis of the release profile of octreotide in a depot formulation, with results in excellent agreement with reported chromatographic assays.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202103040063941ZK.pdf | 579KB | download |