Validação de método para determinação de ácidos orgânicos voláteis em efluentes de reatores anaeróbios empregando cromatografia líquida

Química Nova

Maristela Barnes Rodrigues Cerqueira¹  Adriana Neves Dias¹  Sergiane Souza Caldas¹  Fabrício Butierres Santana¹  Marcelo Gonçalves Montes D'oca¹  Ednei Gilberto Primel¹ 

[1] ,Universidade Federal do Rio Grande Escola de Química e Alimentos Rio Grande RS ,Brasil

关键词: VFAs;    matrix effect;    HPLC-DAD;   

DOI  :  10.1590/S0100-40422011000100029

来源: SciELO

【 摘 要 】

This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r²>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor.

【 授权许可】

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