Produção de micropartículas de alginato contendo Flavobacterium columnare inativada pelo método de emulsão para vacinação de peixes por via oral

Química Nova

Priscila Soares Costa Sacchetin²  Ângela Maria Moraes²  Carlos Augusto Gomes Leal¹  Henrique César Pereira Figueiredo¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Departamento de Processos BiotecnológicosCampinas SP ,Brasil

关键词: alginate microparticles;    emulsion;    fish vaccine;   

DOI  :  10.1590/S0100-40422010000200006

来源: SciELO

【 摘 要 】

Alginate microparticles were prepared by an emulsion method aiming oral controlled release of antigens to fish. The effects of emulsification temperature and impeller type on particle morphology, average diameter, and size distribution were evaluated. Microparticles contaning formalin-killed Flavobacterium columnare cells (a model antigen) were prepared and characterized regarding bacterial release and particle stability when exposed to Nile tilapia (Oreochromis niloticus) typical gastrointestinal conditions. This methodology allowed the production of microparticles containing up to 14.3 g/L of bacterin, stable at a pH range from 2.0 to 9.0 for 12 h and smaller than 35 μm.

【 授权许可】

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