Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Química Nova

Denise Grotto²  Lucas Santa Maria²  Juliana Valentini²  Clóvis Paniz²  Gabriela Schmitt²  Solange Cristina Garcia²  Valdeci Juarez Pomblum¹  João Batista T. Rocha¹  Marcelo Farina¹ 

[1] ,Universidade Federal de Santa Maria Departamento de Análises Cínicas e Toxicológicas Santa Maria RS ,Brasil

关键词: oxidative stress;    MDA;    methodologies;   

DOI  :  10.1590/S0100-40422009000100032

来源: SciELO

【 摘 要 】

Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.

【 授权许可】

CC BY-NC   
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