Extracción y medida de peroxidasa en pulpa de arazá (Eugenia stipitata MC Vaugh)

Química Nova

Carlos Eduardo Narváez-cuenca¹ 

[1] ,Universidad Nacional de Colombia Facultad de Ciencias Departamento de QuímicaBogotá,Colombia

关键词: blanching;    frozen storage;    inactivation;   

DOI  :  10.1590/S0100-40422008000800025

来源: SciELO

【 摘 要 】

A technique for both extraction and activity measurement of peroxidase extracted from arazá (Eugenia stipitata Mc Vaugh) is described. Peroxidase from arazá pulp fruit was extracted using a combination of protein precipitation with acetone and extraction with 50 mM sodium buffer phosphate (pH 6.0). Optimum activity using guaiacol as H-donor was obtained at pH from 5.0 to 6.5, temperature from 60 to 75 °C, H2O2 between 10 to 15 mM and guaiacol from 80 to 160 mM. Thermal inactivation showed a first-order inactivation kinetic. Reactivation was observed when extracts were heated at 80 °C and afterwards incubated at 25 °C.

【 授权许可】

CC BY-NC   
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