Determinação de lamivudina, estavudina e nevirapina, em comprimidos, por cromatografia líquida de alta eficiência

Química Nova

Gisele Rodrigues Da Silva¹  Gustavo Portela Lages¹  Gerson Antônio Pianetti¹  Elzíria De Aguiar Nunan¹  Cristina Duarte Vianna Soares¹  Ligia Maria Moreira De Campos¹ 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos FarmacêuticosBelo Horizonte MG ,Brasil

关键词: antiretroviral;    HPLC;    validation;   

DOI  :  10.1590/S0100-40422006000600002

来源: SciELO

【 摘 要 】

A high performance liquid chromatography method was developed to quantify lamivudine, stavudine and nevirapine combined in tablets. The separation was carried out in less than 10 min using a phosphate buffer of pH 3.0 and acetonitrile (75:25, v/v) as mobile phase, a LiChrospher ODS column and UV detection at 266 nm. The method was linear over the range of 15-135 µg/mL (lamivudine), 4-36 µg/mL (stavudine) and 20-180 µg/mL (nevirapine). The accuracy ranged from 98.56 to 102.04% and intra-day and inter-day precision was less than 1% for the three drugs. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.

【 授权许可】

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