Characterization of rue extract and its potential for controlling rice blast

Pesquisa Agropecuária Brasileira

Karinna Bannach Reis¹  Marcio Vinicius De Carvalho Barros Côrtes¹  Frederico Severino Martins¹  Marta Cristina Corsi De Filippi¹  José Realino De Paula¹  Edemilson Cardoso Da Conceição¹ 

关键词: Magnaporthe oryzae;    Ruta graveolens;    bergapten;    psoralen;    Magnaporthe oryzae;    Ruta graveolens;    bergapteno;    psoraleno;   

DOI  :  10.1590/S0100-204X2015001200001

来源: SciELO

【 摘 要 】

Abstract: The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.

【 授权许可】

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