Hormonal protocols for in vitro production of Zebu and taurine embryos

Pesquisa Agropecuária Brasileira

Carlos Antônio De Carvalho Fernandes¹  Tatimara Maria Miyauchi¹  Ana Cristina Silva De Figueiredo¹  Miller Pereira Palhão¹  Fabiana Cristina Varago¹  Ester Siqueira Caixeta Nogueira¹  Jairo Pereira Neves¹  Tochimara Aparecida Miyauchi¹ 

关键词: Bos indicus;    Bos taurus;    artificial insemination;    follicle growth induction;    follicular wave synchronization;    FSH stimulation;    Bos indicus;    Bos taurus;    inseminação artificial;    indução de crescimento folicular;    sincronização da onda folicular;    estimulação com FSH.;   

DOI  :  10.1590/S0100-204X2014001000008

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated.

【 授权许可】

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