QTL mapping for protein content in soybean cultivated in two tropical environments

Pesquisa Agropecuária Brasileira

Taís Cristina Bastos Soares²  Pedro Ivo Vieira Good-god¹  Fábio Demolinari De Miranda¹  Janaína Bastos Soares¹  Ivan Schuster¹  Newton Deniz Piovesan¹  Everaldo Gonçalves De Barros¹  Maurilio Alves Moreira¹ 

[1] ,Universidade Federal do Espírito Santo Departamento de Zootecnia Alegre ES ,Brazil

关键词: Glycine max;    linkage map;    molecular markers;    recombinant inbred lines;    quantitative trait loci;    Glycine max;    mapa de ligação;    marcador molecular;    linhagem recombinante endogâmica;    locos de características quantitativas;   

DOI  :  10.1590/S0100-204X2008001100012

来源: SciELO

【 摘 要 】

The objectives of this study were to detect quantitative trait loci (QTL) for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL), obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively). Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL were mapped in C2, E and N linkage groups, which explained 14.37, 10.31 and 7.34% of the phenotypic variation of protein content, respectively. For the lines cultivated in Viçosa, two QTL were mapped in linkage groups G and #1, which explained 9.51 and 7.34% of the phenotypic variation of protein content. Based on the mean of the two environments, two QTL were identified: one in the linkage group E (9.90%) and other in the group L (7.11%). In order for future studies to consistently detect QTL effects of different environments, genotypes with greater stability should be used.

【 授权许可】

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