Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao

Pesquisa Agropecuária Brasileira

Thiago Édson Ribeiro Da Silva¹  Luciana Cardoso Cidade¹  Fátima Cerqueira Alvim¹  Júlio Cézar De Mattos Cascardo¹  Marcio Gilberto Cardoso Costa¹ 

[1] ,Universidade Estadual de Santa Cruz Centro de Biotecnologia e Genética Departamento de Ciências Biológicas

关键词: biotechnology;    cacao;    gas exchange;    micropropagation;    morphogenesis;    biotecnologia;    cacau;    troca gasosa;    micropropagação;    morfogênese;   

DOI  :  10.1590/S0100-204X2008001000024

来源: SciELO

【 摘 要 】

The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.

【 授权许可】

CC BY   
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