Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene

Pesquisa Agropecuária Brasileira

Milena Schenkel Homrich¹  Luciane Maria Pereira Passaglia¹  Jorge Fernando Pereira²  Paulo Fernando Bertagnolli²  José Roberto Salvadori²  Marcio Nicolau²  Eliane Kaltchuk-santos¹  Laura Bianchessi Alves¹  Maria Helena Bodanese-zanettini¹ 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Genética Instituto de BiociênciasPorto Alegre RS ,Brazil

关键词: Anticarsia gemmatalis;    Glycine max;    plant resistance to insects;    gene transfer;    Anticarsia gemmatalis;    Glycine max;    resistência de plantas a insetos;    transferência de genes;   

DOI  :  10.1590/S0100-204X2008000700003

来源: SciELO

【 摘 要 】

The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The presence of cry1Ac transgene affected neither the development nor the yield of plants. Cytogenetical analysis showed that transgenic plants presented normal karyotype. In detached-leaf bioassay, cry1Ac plants exhibited complete efficacy against A. gemmatalis, whereas negative controls were significantly damaged. Whole-plant feeding assay confirmed a very high protection of cry1Ac against velvetbean caterpillar, while nontransgenic 'IAS 5' plants and homozygous gusA line exhibited 56.5 and 71.5% defoliation, respectively. The presence of cry1Ac transgene doesn't affect the majority of agronomic traits (including yield) of soybean and grants high protection against A. gemmatalis.

【 授权许可】

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