Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm

Pesquisa Agropecuária Brasileira

Andréa Almeida Carneiro¹  Eliane Aparecida Gomes¹  Claudia Teixeira Guimarães¹  Fernando Tavares Fernandes¹  Newton Portilho Carneiro¹  Ivan Cruz¹ 

[1] ,Embrapa Milho e SorgoSete Lagoas MG ,Brazil

关键词: Spodoptera frugiperda;    entomopathogenic fungi;    RAPD;    rDNA-ITS;    Spodoptera frugiperda;    fungo entomopatogênico;    RAPD;    rDNA-ITS;   

DOI  :  10.1590/S0100-204X2008000400010

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.

【 授权许可】

CC BY   
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