Esterase profile in the postembryonic development of Rhipicephalusmicroplus

Pesquisa Agropecuária Brasileira

Milla Alves Baffi²  Cícero Donizete Pereira¹  Guilherme Rocha Lino De Souza¹  Carlos Roberto Ceron¹  Ana Maria Bonetti¹ 

[1] ,Universidade Estadual de Londrina Dep. de Biologia Geral Lab. de Genética MolecularLondrina PR ,Brazil

关键词: cattle tick;    resistance to acaricides;    tick control;    carrapato bovino;    resistência a acaricidas;    controle de carrapatos;   

DOI  :  10.1590/S0100-204X2007000800016

来源: SciELO

【 摘 要 】

The objective of this work was to analyze the pattern of esterase activity in the development stages of Rhipicephalus microplus by nondenaturing polyacrylamide gel electrophoresis using specific staining for esterase. The electrophoretical results revealed the presence of nine regions displaying esterase activity, stained with both alpha-naphthyl acetate and beta-naphthyl acetate, and classified as alpha-beta-esterase. Stage-specific esterases were found, with the first nymphal and larval stages showing the greatest esterase activity throughout the development. An esterase called EST-4 was detected only in males and was considered sex-specific. There are differences in the esterase profile among the different postembryonic development stages of R. microplus.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License