Validation of microsatellite markers for assisted selection of soybean resistance to cyst nematode races 3 and 14

Pesquisa Agropecuária Brasileira

Marcia Flores Da Silva²  Ivan Schuster¹  João Flávio Veloso Da Silva¹  Adésio Ferreira¹  Everaldo Gonçalves De Barros²  Maurilio Alves Moreira² 

[1] ,Universidade Federal de Viçosa Instituto de Biotecnologia Aplicada à Agropecuária Viçosa MG ,Brazil

关键词: Glycine max;    Heterodera glycines;    soybean breeding;    SCN;    SSR;    MAS;    Glycine max;    Heterodera glycines;    melhoramento de soja;    NCS;    SSR;    SAM;   

DOI  :  10.1590/S0100-204X2007000800011

来源: SciELO

【 摘 要 】

The objective of this work was to validate microsatellite markers associated with resistance to soybean cyst nematode (Heterodera glycines Ichinohe) races 3 and 14, in soybean (Glycine max L.) genotypes, for use in marker-assisted selection (MAS) programs. Microsatellites of soybean linkage groups A2, D2 and G were tested in two populations, and their selection efficiencies were determined. The populations were 65 F2:3 families from Msoy8001 (resistant) x Conquista (susceptible) cross, and 66 F2:3 families of S5995 (resistant) x Renascença (susceptible) cross, evaluated for resistance to races 3 and 14, respectively. Families with female index up to 30% were considered moderately resistant. Markers of A2 and G linkage groups were associated with resistance to race 3. Markers Satt309 and GMENOD2B explained the greatest proportion of phenotypic variance in the different groups. The combinations Satt309+GMENOD2B and Satt309+Satt187 presented 100% selection efficiency. Resistance to race 14 was associated with markers of G linkage group, and selection efficiency in the Satt309+Satt356 combination was 100%. The selection differential obtained by phenotypic and marker assisted selection showed that both can result in similar gains.

【 授权许可】

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