GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange

Pesquisa Agropecuária Brasileira

Fernando Alves De Azevedo²  Francisco De Assis Alves Mourão Filho¹  Evandro Henrique Schinor¹  Luis Gustavo De Paoli¹  Beatriz Madalena Januzzi Mendes¹  Ricardo Harakava¹  Dean W. Gabriel¹  Richard F. Lee¹ 

[1] ,Centro APTA Citros 'Sylvio Moreira'Cordeirópolis SP ,Brazil

关键词: Xylella fastidiosa;    PAL;    citrus variegated chlorosis;    cultivar resistance;    Xylella fastidiosa;    PAL;    clorose variegada dos citros;    resistência varietal;   

DOI  :  10.1590/S0100-204X2006001100008

来源: SciELO

【 摘 要 】

The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants.

【 授权许可】

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