Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants

Pesquisa Agropecuária Brasileira

Eliane Aparecida Gomes¹  Sílvia Neto Jardim¹  Claudia Teixeira Guimarães¹  Isabel Regina Prazeres De Souza¹  Elizabeth De Oliveira¹ 

[1] ,Embrapa Milho e SorgoSete Lagoas MG

关键词: Zea mays;    microorganisms;    spiralin;    mollicutes;    genetic polymorphism;    gene sequence;    Zea mays;    microrganismo;    espiralina;    molicute;    polimorfismo genético;    seqüência genética;   

DOI  :  10.1590/S0100-204X2004000100009

来源: SciELO

【 摘 要 】

The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.

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