Paternity test in "Mangalarga-Marchador" equines by DNA-fingerprinting

Pesquisa Agropecuária Brasileira

Carlos Eduardo AnunciaÇÃo²  Spartaco Astolfi-filho¹ 

[1] ,Universidade Federal de Goiás Dep. de Ciências Fisiológicas Goiânia GO ,[e-mail]

关键词: breeding methods;    molecular cloning;    progeny testing;    horses;    identification;    genetic polymorphism;    métodos de melhoramento;    clonagem molecular;    teste de progênie;    cavalos;    identificação;    polimorfismo;    genético;   

DOI  :  10.1590/S0100-204X2000001000012

来源: SciELO

【 摘 要 】

GC-rich molecular minisatellite probes isolated from the human genome have presented a poor ability for individualization in horses. In this study new DNA sequences were isolated which could be used in paternity tests in horses. Genomic DNA from "Mangalarga-Marchador" horses was treated with restriction enzymes that preferentially digest non-repetitive sequences, so preserving the structure where mini and microsatellites are located. Four clones (S01, S05, S07 and S09) selected from a genomic library screened with a (TG)n oligonucleotide showed similar hybridization profiles generating bands of DNA-fingerprinting type. Using these probes the individualization power obtained was 10-8, which is 10(5)fold higher than that obtained with M13, another GC-rich type probe. All clones were efficient in parentage detection in crossbreedings and presented a 27 bp consensus sequence, GTTTCATTTATTATTCTTTGGAAGAAA, which was repeated 12, 18, 11 and 21 times in clones S01, S05, S07 and S09, respectively.

【 授权许可】

CC BY   
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