Isolation of a cotton NADP(H) oxidase homologue induced by drought stress

Pesquisa Agropecuária Brasileira

Alexandre Lima Nepomuceno²  James Mcd Stewart¹  Derrick Oosterhuis¹  Rickie Turley¹  Morman Neumaier²  JosÉ Renato BouÇas Farias² 

[1] ,Empresa Brasileira de Pesquisa Agropecuária Centro Nacional de Pesquisa de Soja Londrina PR.

关键词: messenger RNA;    gene expression;    genetic transformation;    water deficit;    RNA mensageiro;    expressão gênica;    transformação genética;    déficit hídrico;   

DOI  :  10.1590/S0100-204X2000000700015

来源: SciELO

【 摘 要 】

The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.

【 授权许可】

CC BY   
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