Exon-primed intron-crossing (EPIC) markers as a tool for ant phylogeography

Revista Brasileira de Entomologia

Patrícia R. Ströher²  Chenhong Li¹  Marcio R. Pie² 

[1] ,Universidade Federal do Paraná Departamento de Zoologia Laboratório de Dinâmica Evolutiva e Sistemas ComplexosCuritiba PR ,Brazil

关键词: Formicidae;    molecular markers;    phylogeny;    population genetics;   

DOI  :  10.1590/S0085-56262013005000039

来源: SciELO

【 摘 要 】

Exon-primed intron-crossing (EPIC) markers as a tool for ant phylogeography. Due to their local abundance, diversity of adaptations and worldwide distribution, ants are a classic example of adaptive radiation. Despite this evolutionary and ecological importance, phylogeographical studies on ants have relied largely on mitochondrial markers. In this study we design and test exon-primed intron-crossing (EPIC) markers, which can be widely used to uncover ant intraspecific variation. Candidate markers were obtained through screening the available ant genomes for unlinked conserved exonic regions interspersed with introns. A subset of 15 markers was tested in vitro and showed successful amplification in several phylogenetically distant ant species. These markers represent an important step forward in ant phylogeography and population genetics, allowing for more extensive characterization of variation in ant nuclear DNA without the need to develop species-specific markers.

【 授权许可】

CC BY-NC-ND   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License