Toxicological and ultrastructural analysis of the impact of pesticides used in temperate fruit crops on two populations of Chrysoperla externa (Neuroptera, Chrysopidae)

Revista Brasileira de Entomologia

Alexandre Pinho De Moura²  Geraldo Andrade Carvalho¹  Luciano Veiga Cosme¹  Eduardo Alves¹  Marcos Botton¹  Patrícia Sobral Silva¹ 

[1] ,Empresa Brasileira de Pesquisa Agropecuária Embrapa Hortaliças Laboratório de EntomologiaBrasília DF ,Brasil

关键词: Green lacewings;    integrated pest management;    natural enemy;    scanning electronic microscopy;    side-effects;    Crisopídeos;    efeitos adversos;    manejo integrado de pragas;    microscopia eletrônica de varredura;    inimigo natural;   

DOI  :  10.1590/S0085-56262011005000046

来源: SciELO

【 摘 要 】

This study aimed to evaluate the effects of (g a.i. L-1) abamectin (0.02), carbaryl (1.73), sulphur (4.8), fenitrothion (0.75), methidathion (0.4), and trichlorfon (1.5) on the survival of larvae and pupae, on the oviposition of adults and hatching of eggs from treated Chrysoperla externa third-instar larvae from two different populations (Bento Gonçalves and Vacaria, Rio Grande do Sul State, Brazil). Morphological changes caused by abamectin to eggs laid by C. externa from Vacaria population were evaluated by mean of ultrastructural analysis. The pesticides were applied on glass plates. Distilled water was used as control. For the evaluation of larvae mortality, a fully randomized experimental design in a 2 x 7 (two populations x seven treatments) factorial scheme was used, whereas for the effects of the compounds on oviposition capacity and egg viability, a 2 x 4 factorial scheme was used. Carbaryl, fenitrothion, and methidathion caused 100% mortality of larvae. Abamectin reduced the hatching of eggs from treated third-instar larvae of both populations; however, this pesticide presented highest toxicity on insects from Vacaria. The ultrastructural analysis showed that abamectin caused malformations in micropyle and in chorion external surface of C. externa eggs. Based in the total effect (E), carbaryl, fenitrothion, and methidathion are harmful to C. externa; trichlorfon is harmless to third-instar larvae, while abamectin and sulphur are harmless and slightly harmful to third-instar larvae from Bento Gonçalves and Vacaria, respectively.

【 授权许可】

CC BY-NC-ND   
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