DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis

Memórias do Instituto Oswaldo Cruz

Karine M Forster¹  Daiane D Hartwig¹  Thaís L Oliveira¹  Kátia L Bacelo¹  Rodrigo Schuch¹  Marta G Amaral¹  Odir A Dellagostin¹ 

关键词: leptospirosis;    Leptospira;    LigBrep;    subunit vaccine;    DNA vaccine;    prime-boost;   

DOI  :  10.1590/0074-02760150222

来源: SciELO

【 摘 要 】

Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.

【 授权许可】

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