Anti-Mycobacterium tuberculosis activity and cytotoxicity of Calophyllum brasiliense Cambess (Clusiaceae)

Memórias do Instituto Oswaldo Cruz

Claudia Terencio Agostinho Pires¹  Mislaine Adriana Brenzan¹  Regiane Bertin De Lima Scodro¹  Diógenes Aparício Garcia Cortez¹  Luciana Dias Ghiraldi Lopes¹  Vera Lucia Dias Siqueira¹  Rosilene Fressatti Cardoso¹ 

关键词: Mycobacterium tuberculosis;    Calophyllum brasiliense;    coumarin;    cytotoxicity - 3-(4;    5-dimethyl thiazol-2-yl)-2;    5-diphenyl tetrazolium bromide;   

DOI  :  10.1590/0074-0276130323

来源: SciELO

【 摘 要 】

We evaluated the in vitro anti-Mycobacterium tuberculosis activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of Calophyllum brasiliense. Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 ± 2.6 and 8.24 ± 1.1 µg/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-M. tuberculosis H37Rv activity of 62.5 and 125 µg/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 µg/mL against M. tuberculosis H37Rv and clinical isolates. Compound (3) showed no activity against M. tuberculosis H37Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of C. brasiliense against M. tuberculosis.

【 授权许可】

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