Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Memórias do Instituto Oswaldo Cruz

Gardenia Braz Figueiredo Carvalho¹  Rosiane Aparecida Da Silva-pereira²  Lucila Grossi Gonçalves Pacífico¹  Cristina Toscano Fonseca¹ 

[1] ,Fiocruz Instituto de Pesquisas René Rachou Laboratório de Parasitologia Celular e Molecular

关键词: Schistosoma mansoni;    diagnosis;    antigen candidate;    bioinformatics analysis;   

DOI  :  10.1590/S0074-02762011000700009

来源: SciELO

【 摘 要 】

The development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.

【 授权许可】

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