Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon

Memórias do Instituto Oswaldo Cruz

Freddy Ruiz²  Yvonne-marie Linton¹  David J Ponsonby¹  Jan E Conn¹  Manuela Herrera¹  Martha L Quiñones¹  Iván D Vélez¹  Richard C Wilkerson² 

[1] ,Walter Reed Army Institute of Research Division of Entomology Maryland,USA

关键词: Anopheles dunhami;    COI barcodes;    ITS2;    topotypic;    Brazil;    Colombia;   

DOI  :  10.1590/S0074-02762010000700010

来源: SciELO

【 摘 要 】

The presence of Anopheles (Nyssorhynchus) dunhami Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, Brazil. An. dunhami was identified through retrospective correlation of DNA sequences following misidentification as Anopheles nuneztovari s.l. using available morphological keys for Colombian mosquitoes. That An. dunhami occurs in Colombia and also possibly throughout the Amazon Basin, is of importance to vector control programs, as this non-vector species is morphologically similar to known malaria vectors including An. nuneztovari, Anopheles oswaldoi and Anopheles trinkae. Species identification of An. dunhami and differentiation from these closely related species are highly robust using either DNA ITS2 sequences or COI DNA barcode. DNA methods are advocated for future differentiation of these often sympatric taxa in South America.

【 授权许可】

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