Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients

Memórias do Instituto Oswaldo Cruz

Isabela Gama Sardella¹  Mahavir Singh²  Susanne Kumpfer²  Rafael Ribeiro Heringer¹  Maria Helena Féres Saad¹  Marzia Puccioni Sohler¹ 

[1] ,Lionex Diagnostics and TherapeuticsBraunschweig,Germany

关键词: tuberculosis;    cerebrospinal fluid;    ELISA;    meningoencephalitis tuberculosis;    Mycobacterium tuberculosis;    immune response;   

DOI  :  10.1590/S0074-02762010000500022

来源: SciELO

【 摘 要 】

To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2%, 47.4%; 95%, 93.7%; 40%, 98% and 28.4%, 97.1%, respectively). However, while grey zone was 12.7% and 6%, respectively, lowering sensitivity but maintains high specificity (> 95%). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License