Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon

Memórias do Instituto Oswaldo Cruz

Andrezza Campos Chagas²  Jansen Fernandes Medeiros¹  Spartaco Astolfi-filho¹  Victor Py-daniel¹ 

[1] ,Instituto de Pesquisa René Rachou-Fiocruz Laboratório de Entomologia Médica Belo Horizonte MG ,Brasil

关键词: black flies;    salivary glands;    anticoagulant;    onchocerciasis;   

DOI  :  10.1590/S0074-02762010000200011

来源: SciELO

【 摘 要 】

In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 μg ± 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ages analyzed (12 h, 24 h, 48 h and 72 h following emergence). Coagulation bioassays showed that the SGHs had activities that interacted at all levels of coagulation (the intrinsic, extrinsic and common pathways), by extending the plasma recalcification time, prothrombin time, thrombin time. This is the first report on the activity of salivary gland proteins from the main vector of onchocerciasis in Brazil. We also suggest detailed studies on the morphology and function of the salivary glands in order to understand the role of these proteins in host/vector interactions.

【 授权许可】

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