α-N-acetylglucosamine-linked O-glycans of sialoglycoproteins (Tc-mucins) from Trypanosoma cruzi Colombiana strain

Memórias do Instituto Oswaldo Cruz

Adriane R Todeschini²  Eliane G De Almeida²  Orlando A Agrellos²  Christopher Jones¹  José O Previato²  Lucia Mendonça-previato² 

[1] ,Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Instituto de Biofísica Carlos Chagas FilhoRio de Janeiro RJ ,Brasil

关键词: Trypanosoma cruzi;    NMR spectroscopy;    glycoconjugate;    Colombiana strain;    mucins;    sialic acid;   

DOI  :  10.1590/S0074-02762009000900035

来源: SciELO

【 摘 要 】

Trypanosoma cruzi sialoglycoproteins (Tc-mucins) are mucin-like molecules linked to a parasite membrane via a glycosylphosphatidylinositol anchor. We previously determined the structures of Tc-mucin O-glycan domains from several T. cruzi strains and observed significant differences among them. We now report the amino acid content and structure of Tc-mucin O-glycan chains from T. cruzi Colombiana, a strain resistant to common trypanocidal drugs. Amino acid analysis demonstrated the predominance of threonine residues (42%) and helped to identify the O-glycans as belonging to a Tc-mucin family that contain a ²-galactofuranose (²-Galf) residue attached to an α-N-acetylglucosamine (α-GlcNAc) O-4, with the most complex glycan, a pentasaccharide-GlcNAc-ol with a branched trigalactopyranose chain, on the GlcNAc O-6. The presence of ²-Galf on O-glycans from T. cruzi Colombiana mucins supports the use of glycosylation as a phylogenetic marker for the classification of Colombiana in the T. cruzi I group.

【 授权许可】

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