Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Memórias do Instituto Oswaldo Cruz

Luciana I Gomes²  Letícia Hs Marques²  Martin J Enk¹  Paulo Marcos Z Coelho¹  Ana Rabello² 

[1] ,Laboratório de Pesquisas Clínicas

关键词: schistosomiasis;    molecular diagnosis;    faecal samples;    polymerase chain reaction;   

DOI  :  10.1590/S0074-02762009000800021

来源: SciELO

【 摘 要 】

A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.

【 授权许可】

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