Use of a saline gradient for the diagnosis of schistosomiasis

Memórias do Instituto Oswaldo Cruz

Paulo Marcos Z Coelho²  Arnon D Jurberg¹  Áureo A Oliveira²  Naftale Katz² 

[1] ,Fiocruz Centro de Pesquisas René Rachou Laboratório de EsquistossomoseBelo Horizonte MG ,Brasil

关键词: Schistosoma mansoni;    diagnosis;    faeces;   

DOI  :  10.1590/S0074-02762009000500010

来源: SciELO

【 摘 要 】

The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3% saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a signifi-cantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10% formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences.

【 授权许可】

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