Oxidative stress response to menadione and cumene hydroperoxide in the opportunistic fungal pathogen Candida glabrata

Memórias do Instituto Oswaldo Cruz

Mayra Cuéllar-cruz¹  Irene Castaño¹  Omar Arroyo-helguera¹  Alejandro De Las Peñas¹ 

[1] ,Instituto Potosino de Investigación Científica y Tecnológica División de Biología Molecular San Luis Potosí SLP ,México

关键词: Candida glabrata;    menadione;    cumene;    CTA1;    Candida albicans;    Saccharomyces cerevisiae;   

DOI  :  10.1590/S0074-02762009000400020

来源: SciELO

【 摘 要 】

Candida glabrata is an opportunistic fungal pathogen that can cause severe invasive infections and can evade phagocytic cell clearance. We are interested in understanding the virulence of this fungal pathogen, in particular its oxidative stress response. Here we investigated C. glabrata, Saccharomyces cerevisiae and Candida albicans responses to two different oxidants: menadione and cumene hydroperoxide (CHP). In log-phase, in the presence of menadione, C. glabrata requires Cta1p (catalase), while in a stationary phase (SP), Cta1p is dispensable. In addition, C. glabrata is less resistant to menadione than C. albicans in SP. The S. cerevisiae laboratory reference strain is less resistant to menadione than C. glabrata and C. albicans; however S. cerevisiaeclinical isolates (CIs) are more resistant than the lab reference strain. Furthermore, S. cerevisiae CIs showed an increased catalase activity. Interestingly, in SP C. glabrata and S. cerevisiae are more resistant to CHP than C. albicans and Cta1p plays no apparent role in detoxifying this oxidant.

【 授权许可】

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