Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis

Memórias do Instituto Oswaldo Cruz

Zélia Maria Profeta Luz¹  Alexandre Rotondo Da Silva¹  Fernanda De Oliveira Silva¹  Rachel B Caligiorne¹  Edward Oliveira¹  Ana Rabello¹ 

[1] ,Fiocruz Instituto René Rachou Centro de Referência em LeishmaniosesBelo Horizonte MG ,Brasil

关键词: puncture biopsy;    cutaneous leishmaniasis;    diagnosis;    health services;   

DOI  :  10.1590/S0074-02762009000100010

来源: SciELO

【 摘 要 】

The detection of Leishmania spp. in skin lesion aspirates, using a puncture technique, was evaluated in 76 patients with cutaneous leishmaniasis (CL) who were referred to a Leishmaniasis Reference Centre in Brazil. CL was defined based on skin lesions suggestive of the disease and on a positive result of the Montenegro skin test or Giemsa-stained imprints of biopsy fragments. The aspirates were cultured using a vacuum tube device containing culture medium and evaluated for the presence of Leishmania spp. The biphasic medium culture was examined once a week for three weeks. Promastigotes were observed in 53/76 (69.7%) cultures. Stained smears from 60 of the 76 patients were evaluated using PCR-RFLP to detect the conserved minicircle region of Leishmania spp. and to classify the parasite. Of these patients, 45 (75%) showed positive results in aspirate culture and 15 presented negative results. The PCR was positive in 80% (53/60) samples. The PCR-RFLP profile was determined in 49 samples, of which 45 (92%) showed a pattern compatible with Leishmania (Viannia) braziliensis. The aspirate culture is a sensitive and feasible method for diagnosing CL and may be routinely adopted by health services for L. (V.) braziliensis isolation and identification.

【 授权许可】

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