Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Memórias do Instituto Oswaldo Cruz

Ximena Carolina Pulido²  Gerardo Pérez¹  Gustavo Adolfo Vallejo² 

[1] ,Universidad del Tolima Facultad de Ciencias Laboratorio de Investigaciones en Parasitología TropicalIbagué,Colombia

关键词: Rhodnius prolixus;    Trypanosoma rangeli KP1(+);    Trypanosoma rangeli KP1(-);    trypanolytic factor;    vectorial ability;    insect immunity;   

DOI  :  10.1590/S0074-02762008000200008

来源: SciELO

【 摘 要 】

Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.

【 授权许可】

CC BY   
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